The 1.8 million sq ft project was announced by Mayor Joe Anderson and will be located on the former Archbishop Blanch school site at Minshull Street.
Features include Grade A office space along with units focused on the life sciences sector, and a new rail station with access to the underground network and the national high speed rail network via Lime Street Station.
With the scheme forming a key part of the Knowledge Quarter Mayoral Development Zone, it is estimated up to 10,000 jobs could be created.
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Mayor Anderson said: "The extension of our Knowledge Quarter is a game-changer in terms of the high-quality jobs we will be able to bring into the city in hi-tech, 21st century industries.
"This will help us to develop our Knowledge Quarter as we work with all our partners, including the University of Liverpool and the proposed health campus at the Royal Hospital to make the city a beacon for investment in this and similar sectors.
"We know there is a real North/South gap in investment around life sciences that sees an overwhelming concentration of jobs and investment head south. However this landmark scheme provides the basis for Liverpool to now become a national and international destination for investment in life and medical sciences, bringing with it thousands of new, high-skill, high-wage jobs."
Phase One of the project is due to begin on-site in 2017.
(LM/MH)
